【文獻(xiàn)標(biāo)題】Inhibited autophagy impairs systemic nutrient metabolism in Nile tilapia

【作者】Si-Lan Han，Jing Wang，Yu-Xue Zhang，et.al

【作者單位】華東師范大學(xué)（East China Normal University）

【文獻(xiàn)中引用產(chǎn)品】

魚(yú)胰島素（Insulin）ELISA試劑盒

【關(guān)鍵詞】Autophagy，Metabolism，Homeostasis，Inflammation，Nile tilapia

【DOI】doi.org/10.1016/j.cbpa.2019.06.021

【影響因子(IF)】4.0

【出版期刊】《Comparative Biochemistry and Physiology, Part A》

【產(chǎn)品原文引用】

At the end of trial, all fish were fasted overnight, six fish of each group were euthanized (MS-222 at 20 mg/L) and sampled to collect tissues to measure the molecular, protein and biochemical indexes.Hepatic triglyceride (TG), glycogen, malondialdehyde (MDA), superoxide dismutase (SOD), and serum TG, free fatty acid (FFA), and glucose were assessed by commercial kits (Jiancheng Biotech Co. China).The serum insulin was detected by ELISA kits (Hengyuan Biotech Co.China). Briefly, the total lipid of the whole fish body, liver and muscle was extracted by using chloroform/methanol (2:1, v/v) as previously described (Bligh and Dyer, 1959). Briefly, the samples were homogenized in the mixed chloroform-methanol 2:1 (vol/vol), and the samples were stored at 4 °C for 24-h extraction. Afterwards, the chloroform phase was carefully moved to a clean glass tube and dried using nitrogen, and the extracted total lipid was weighed and recorded.Whole fish protein and muscle protein were measured by Kjeltec? 8200(FOSS, Sweden).